|Year : 2019 | Volume
| Issue : 2 | Page : 66-72
Recent insights in atopic dermatitis pathogenesis, treatment, and disease impact
Madison Nguyen1, Adrian Pona1, Sree S Kolli1, Steven R Feldman2, Lindsay C Strowd1
1 Department of Dermatology, Center for Dermatology Research, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA
2 Department of Dermatology, Center for Dermatology Research; Department of Pathology; Department of Social Sciences and Health Policy, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA
|Date of Web Publication||26-Jul-2019|
Dr. Adrian Pona
Department of Dermatology, Center for Dermatology Research, Wake Forest School of Medicine, Winston-Salem, North Carolina 27104
Source of Support: None, Conflict of Interest: None
Atopic dermatitis (AD) is the most common inflammatory skin condition impacting patient quality of life. Although AD is widely studied, investigators have explored recent advancements in AD pathogenesis, treatment, and disease impact. Therefore, this article summarizes recent advancements in AD pathogenesis, treatments, and disease impact on patient quality of life. A PubMed search was conducted using the keywords: “atopic dermatitis AND pathogenesis,” “atopic dermatitis AND microbiota,” “atopic dermatitis AND dupilumab,” “atopic dermatitis AND JAK$ inhibitors,” and “atopic dermatitis AND quality of life.” Epidermal barrier dysfunction and immune dysregulation play a key role in the pathogenesis of AD. Although most AD patients express a filaggrin mutation, such mutation alone does not predict disease severity. Immune dysregulation is characterized by T-helper-2 responses in acute AD and Th1 responses in chronic AD. Skin microbiota abnormalities and sweat exacerbate symptomatology. Dupilumab targets the interleukin (IL)-4Rα and is the only Food and Drug Administration-approved biologic that effectively treats AD. Newer alternative agents for AD treatment include IL-12 and IL-23 inhibitors, IL-31R inhibitors, and JAK inhibitors. AD patients have increased anxiety, depression, and sleep disorders (P < 0.001) and skin pain (P= 0.02) compared to control. AD is influenced by the epidermis, immune system, genetics, microbiota, sweat, and environment. AD has lasting impacts on patients' mental and physical health. Dupilumab is an effective biologic for treating the condition.
Keywords: Baricitinib, comorbidity, dupilumab, pathogenesis
|How to cite this article:|
Nguyen M, Pona A, Kolli SS, Feldman SR, Strowd LC. Recent insights in atopic dermatitis pathogenesis, treatment, and disease impact. J Dermatol Dermatol Surg 2019;23:66-72
|How to cite this URL:|
Nguyen M, Pona A, Kolli SS, Feldman SR, Strowd LC. Recent insights in atopic dermatitis pathogenesis, treatment, and disease impact. J Dermatol Dermatol Surg [serial online] 2019 [cited 2020 Feb 28];23:66-72. Available from: http://www.jddsjournal.org/text.asp?2019/23/2/66/263607
| Introduction|| |
Atopic dermatitis (AD) is a chronic skin condition affecting children and adults.,,, While 70% of individuals outgrow AD before adolescence, 30% of cases persist into adulthood., AD is characterized by an imperfect cutaneous barrier and dysregulated inflammation. Although the precise role remains unknown, inflammation in acute AD is attributed to T-helper (Th)-2 cells and increased predisposition to skin infections.,, AD patients have higher rates of mood disorders, sleep disorders, and less work productivity. Approximately 50% of children with AD report that their disease negatively impacts their quality of life.
Mild AD is typically managed with topical corticosteroids. More severe cases of AD require additional oral immunosuppression with cyclosporine, methotrexate, azathioprine, and mycophenolate or immunotherapy. Long- term treatment of AD with traditional immunosuppressive agents has adverse side effects that may limit their use. Thus, additional safer treatment options are needed for AD.
Targeted biologic agents are in development to treat AD resistant to systemic immunosuppression. Currently, dupilumab is a Food and Drug Administration (FDA)-approved biologic for moderate-to-severe adult AD. Other biologic agents under study include lebrikizumab, nemolizumab, and Janus kinase (JAK) inhibitors. Since new research in recent years has explored clinical manifestations, social effects, and therapies for AD, the purpose of this review was to gather insight into recent advancements in AD pathogenesis, treatment, and impact on the quality of life.
| Methods|| |
A PubMed search was conducted using the keywords “atopic dermatitis AND pathogenesis,” “atopic dermatitis AND microbiota,” “atopic dermatitis AND dupilumab,” “atopic dermatitis AND quality of life,” and “atopic dermatitis AND JAK$ inhibitors” [Figure 1]. AD pathogenesis articles were selected based on citation frequency and relevance.
|Figure 1: Flowchart of atopic dermatitis PubMed search. AD: Atopic dermatitis, CT: Clinical trial, CR: Case report, CS: Case series|
Click here to view
| Results|| |
Pathogenesis of atopic dermatitis
The pathogenesis of AD is influenced by epidermal barrier dysfunction and immune system dysregulation., Epidermal barrier dysfunction initiates the development of AD whereas immune system dysregulation further contributes to disease development. Skin microbiota, sweat, and the environment also contribute.,
Epidermal barrier dysfunction
AD patients have decreased barrier-stabilizing proteins and lipids. In AD patients, keratinocytes in the stratum corneum are supported with loricrin (LOR), involucrin (IVL), and particles rich in proline. Cells exposed to tumor necrosis factor-alpha and interleukin (IL)-4 are subject to inflammation and downregulation of LOR and filaggrin (FLG)., Decreased lipid synthesis further contributes to impaired epidermis in AD patients. In AD lesional skin, expression of the enzyme stearoyl-CoA desaturase is upregulated among viable cell layers. This results in increased unsaturated fatty acids and abnormal keratinization in the epidermis.
FLG mutation is the most common identified genetic factor in AD development. FLG2 mutations within a cohort of 60 African American participants are associated with more persistent AD (P = 0.004). Although loss of function mutation in FLG is linked to earlier and more severe AD, patients can develop AD without the mutation. FLG mutations are found in 15%–50% of AD patients whereas 40% of FLG mutation carriers do not develop AD.
Impaired skin barrier predisposes to Staphylococcus aureus colonization and herpes simplex virus infection.S. aureus adhesion occurs via fibronectin and fibrinogen. S. aureus releases an exotoxin called α-toxin that enhances T-cell proliferation and IL-22 secretion. About 70% of S. aureus strains produce staphylococcal enterotoxins (SEs) A, B, and C and toxic shock syndrome toxin-1 (TSST-1). These exotoxins function as superantigens that penetrate the epidermis, increase inflammation, and degrade the already deficient epidermal barrier.,S. aureus strains from AD skin have increased T-cell proliferation, increased IL-2, and decreased interferon-gamma (IFN-γ) compared to non-AD skin.
Immune system dysregulation
AD was widely characterized by cytokine-producing Th2 cells, although recent evidence suggests that AD involves a complex interplay between Th1 and Th2 cells., Th2 cells are primarily involved in acute AD whereas Th1 cells are primarily involved in chronic AD. As acute AD begins, Th2, Th17, and Th22 cytokines are released., However, as AD progresses to a chronic state, Th1 mounts an additional immune response.
Th2 cells produce pro-inflammatory cytokines and IL-4, IL-5, IL-13, and IL-31. IL-4 induces the differentiation of Th2 cells to produce additional IL-4. Increased Th2 products downregulate Th1 cell production. IL-4 downregulates genes encoding FLG, LOR, and IVL and increases fibronectin, increasing the ability for S. aureus to adhere to the skin.,
IL-4 and IL-13 inhibit keratinocyte differentiation and influence barrier dysfunction in patients with AD. IL-13 induces the migration of CD4+ T-cells, mast cells, eosinophils, and macrophages in the dermis and skin remodeling secondary to inflammation. Both IL-4 and IL-13 induce periostin expression that contributes to additional tissue remodeling in patients with chronic AD.
IL-5 functions as a growth factor for B-cells and supports eosinophil growth and proliferation. In a case–control study of 1120 individuals, IL-5 serum levels were higher in AD lesional skin compared to control (P < 0.05). However, a monoclonal antibody against IL-5 was ineffective in AD. IL-31R enhances pruritus in AD.,
IL-17 and IL-22 are increased in acute and chronic AD. Acute AD is associated with higher amounts of IL-22. In acute AD, IFN-γ production is inhibited by immunoglobulin (IgG). IFN-γ production increases in chronic AD. IFN-γ induces macrophages that secrete pro-inflammatory cytokines and growth factors. Thus, macrophages have a role in chronic AD. Since IFN-γ is a Th1-derived cytokine, this supports evidence for Th1 involvement in chronic AD. Furthermore, IFN-γ reduces IL-4 and IL-13 expression in chronic AD, suggesting a complex interaction between Th1 and Th2 in AD.
Keratinocytes produce antimicrobial peptides (AMPs) including defensins, cathelicidins, calprotectins, and dermcidins. AMPs enhance the innate response resulting in neutrophil, monocyte, and T-cell recruitment in the epidermis. Decreased or absent AMPs contribute to cutaneous infection in AD patients. Calprotectin is comprised of damage-associated molecular pattern (DAMP) molecules S100A8 and S100A9. Upregulation of S100A8 and S100A9 expression in response to IL-17A suggests the involvement of the DAMP-mediated inflammation pathway in AD. Dermcidin peptides are reduced in AD patients, resulting in decreased skin protection and greater susceptibility to bacterial colonization.
Human β-defensins (hBDs) are located on epithelial surfaces and activate upon injury or infection. However, Th2 cytokines downregulate hBD expression as the inflammatory cascade is activated. In AD, impaired hBD expression contributes to bacterial and viral susceptibility. hBD-2 and hBD-3 are located in the upper epidermis and have antibacterial properties against S. aureus and herpes simplex virus that commonly colonize AD. IL-4 and IL-13 exacerbate AD by downregulating hBD-2 and hBD-3.,
The influence of microbiota
AD skin generally expresses low bacterial diversity with greater abundance of S. aureus and Staphylococcus epidermidis compared to other genera such as Propionibacterium. Microbiome diversity is decreased in AD skin compared to non-AD skin in both young children (P < 0.001) and adults-teenagers (P = 0.013). In children and adults-teenagers, Staphylococcus was more abundant in AD skin compared to non-AD healthy skin. However, Streptococcus and Propionibacterium were decreased in AD skin compared to non-AD and healthy skin in children and adults-teenagers. This suggests skin microbiota changes with age.
S. aureus threatens AD patients due to multiple virulence factors. Phenol-soluble modulins (PSMs) are a staphylococcal virulence factor that forms α-helical structures. These structures form cytotoxic pores in keratinocytes. PSMs also enhance S. aureus biofilm., Additional virulence factors include microbial surface components recognizing adhesive matrix and superantigens. Superantigens include SEs, SE-like superantigens, and S. aureus TSST-1. About 57% of AD patients' S. aureus skin colonization isolates produced superantigens compared to 33% of control. Furthermore, S. aureus penetration through the epidermis directly correlates with increased IL-4, IL-13, and IL-22 and decreased levels of cathelicidin.
In a randomized, placebo-controlled, single-blinded clinical trial of 21 patients with AD and 14 healthy controls, application of topical corticosteroid fluticasone for 4 weeks normalized the microbiome in childhood AD. In children of age 3 months–5 years with moderate-to-severe AD, baseline densities of total bacteria and Staphylococcus were higher at the worst AD lesional sites compared to nonlesional (P = 0.001) and control (P < 0.001) skin. After fluticasone treatment, lesional bacterial communities resembled nonlesional AD skin yet differed from controls (P < 0.0001). Normalization of skin microbiome with topical corticosteroid treatment is evident through the restoration of microbial diversity and replacement of S. aureus by Corynebacterium, Prevotella, and Acinetobacter.
Substances contained in sweat maintain skin integrity. Sweat contains antimicrobial factors and protease inhibitors. AD patients have abnormal sweat composition and volume.
Sweat from patients in acute AD has increased glucose concentration compared to sweat from healthy controls and patients with chronic AD (P = 0.0331). Sweat glucose concentration positively correlated with severity.
Keratin-plugged sweat pores in AD skin contribute to hypohidrosis, called “sweat retention syndrome.”, Sweat retention may lead to heat retention, xerosis, and increased risk of infection. Histamine can also influence sweating. Since acetylcholine induces perspiration, histamine can suppress perspiration by phosphorylating sweat gland secretory cells consequently inhibiting of acetylcholinergic sweating.
Children exposed to smoke can absorb harmful metabolites, including nicotine. One specific metabolite of nicotine, called cotinine, was elevated in urine samples of 1669 toddlers exposed to tobacco smoke. Children exposed to environmental tobacco smoke had a greater risk of developing AD compared to controls (odds ratio [OR]: 1.97; 95% confidence interval [CI]: 1.23–3.16). Therefore, tobacco smoke could contribute to the development of AD.
Treatment of atopic dermatitis
Topical corticosteroids and topical calcineurin inhibitors are FDA-approved for the primary treatment of AD.,,, Since topical corticosteroids and topical calcineurin inhibitors have been thoroughly studied, this review will discuss novel topical and biologic agents for the treatment of AD.
Phosphodiesterase (PDE) enzymes are targeted by topical AD treatments. PDE-4 is expressed in inflammatory cells. Crisaborole is a topical PDE-4 inhibitor. A study of 1522 participants aged 2 years and older was randomized to crisaborole or vehicle in two identically designed, double-blind, vehicle-controlled studies. More participants in the topical 2% crisaborole group reported a clear score compared to placebo after 28 days of twice-daily application (crisaborole, 51.7%; placebo, 40.6%; P < 0.001).
Dupilumab is a human monoclonal antibody against IL-4Rα. It blocks IL-4 and IL-13 signaling pathways. IL-4Rα is found on inflammatory cells such as keratinocytes, T-cells, dendritic cells, and eosinophils. IL-4 and IL-13 induce IgE class switching, promote Th2 survival, recruit eosinophils, and mediate pruritus. IL-4 and IL-13 inhibit keratinocyte terminal differentiation and AMP production. Dupilumab decreases Th2 inflammatory cytokines and reverses epidermal barrier dysfunction. Dupilumab targets products of Th2 cells that are upregulated in chronic AD. These products are IL-5, IL-13, IL-10, and IL-31. The cellular targets of dupilumab include T-cells, B-cells, mast cells, basophils, and dendritic cells. Dupilumab is the first FDA-approved biologic for the treatment of moderate-to-severe AD. The recommended initial dose is 600 mg (two 300 mg subcutaneous injections) followed by 300 mg every other week.
The Eczema Area and Severity Index (EASI) was used in a randomized, placebo-controlled, Phase IIb clinical trial of 379 adult patients with moderate-to-severe AD to evaluate dupilumab efficacy. The largest decrease in EASI was in the group receiving dupilumab once per week (300 mg, 73.7%) and once every 2 weeks compared to placebo (300 mg, 68.2%; placebo, 18.1%) at week 16 (P < 0.0001). A dose-dependent relationship was reported.
In two randomized, double-blind, placebo-controlled, Phase III clinical trials, dupilumab improved symptoms in 671 moderate-to-severe AD patients 18 years and older. More participants who received 300 mg subcutaneous dupilumab once per week (38%) or once every 2 weeks (37%) than placebo (10%) had an Investigator Global Assessment Score of 0–1 or at least a 2-point decrease from baseline at week 16 (P < 0.001). They also reported improved symptoms of anxiety, depression, and overall quality of life compared to placebo. Clinical improvement with dupilumab was observed in as little as 4 weeks of treatment. Another 12-week randomized study reported that a higher proportion of participants in the dupilumab group achieved EASI-50 compared to the placebo group at week 12 (dupilumab 300 mg, 85%; placebo, 35%; P < 0.001). Finally, a 16-week randomized double-blind, placebo-controlled study using dupilumab improved clinical responses in 452 participants. EASI improvement was greater in all dupilumab groups compared to placebo (300 mg once a week, 74%; 300 mg every 2 weeks, 68%; 300 mg every 4 weeks, 64%; 200 mg every 2 weeks, 65%; 100 mg every 4 weeks, 45%; placebo, 18%; P < 0.0001) at week 16.
A meta-analysis reported a decreased incidence of skin infection in the dupilumab group compared to the placebo group (dupilumab, 6.7%; placebo, 13.3%; P < 0.00001). Rates of upper respiratory tract infections were similar between the dupilumab (6.5%) and placebo (5.4%) group (P = 0.11). Nasopharyngitis incidence was similar between the dupilumab (15.7%) and placebo (13.9%) groups (P = 0.55). There was a higher incidence of conjunctivitis in the dupilumab group (8.0%) compared to the placebo (3.6%) (P < 0.0001). Although in one study the rate of herpes virus infection was similar between the dupilumab (6.1%) and placebo groups (5.2%; P = 0.30), there was an increased rate of herpes viral infections in dupilumab participants compared to placebo (8% vs. 2%) in a different study.,
Ustekinumab is a human monoclonal antibody that targets the p40 subunit of IL-12 and IL-23. In two different randomized, placebo-controlled Phase II trials, ustekinumab failed to achieve a significant improvement in the EASI score.
Nemolizumab is a humanized antibody against the IL-31R. IL-31R is found on keratinocytes and monocytes and is associated with AD-associated pruritus. Nemolizumab improved pruritus in 264 moderate-to-severe AD patients in a randomized, double-blind, placebo-controlled Phase II trial. All nemolizumab groups achieved significantly greater EASI improvements compared to placebo (0.1 mg/kg every 4 weeks, 23%; 0.5 mg/kg every 4 weeks, 42.3%; 2.0 mg/kg every 4 weeks, 40.9%; placebo, 26.6%) at week 12. Patients in the highest dosing group also reported a 59%–63% reduction in sleep disturbance.
Omalizumab is a humanized IgG1 monoclonal antibody that binds to IgE, consequently inhibiting mast cell or basophil activation. In a study of adults with severe AD and elevated IgE, omalizumab had a significant EASI improvement in only two of ten patients. Potential for clinical use of omalizumab is restricted by a higher incidence rate of cardiovascular events in AD patients receiving omalizumab (13.4/1000 person-years; 95% CI: 11.6–15.4) compared to controls (8.1/1000 person-years; 95% CI: 6.5–10.1).
JAK/signal transducer and activator of transcription (STAT) signaling is associated with AD pathogenesis. In a study of 69 adult patients with mild-to-moderate AD, adults treated with 2% tofacitinib ointment had greater EASI improvement compared to placebo (tofacitinib twice-daily applications, 81.7%; placebo, 29.9%; P < 0.001) at week 4. Participants reported alleviation of itching as early as day 2. JTE-052 is a newer topical ointment that inhibits JAK. JTE-052 targets JAK1, JAK2, JAK3, and tyrosine kinase 2. [Please add citation for PMID: 28960254] Through improvement of skin barrier dysfunction and suppression of IL-31 induced pruritus, JTE-052 consequently restores the skin barrier function and suppresses inflammation. [Please add citation for PMID: 26115905]. In a randomized, vehicle-controlled, Phase II clinical study, 327 participants were randomized (2:2:2:2:1:1) to receive JTE-052 ointment at 0.25%, 0.5%, 1%, 3%, the control ointment, or tacrolimus 0.1% twice daily. All JTE-052 groups had improved EASI scores compared to placebo at week 4 (0.25%, 41.7%; 0.5%, 57.1%; 1%, 54.9%; 3%, 72.9%; placebo, 12.2%; P < 0.001). A study of 6 participants treated with oral tofacitinib citrate for 8–29 weeks reported improvement in AD-related pruritus and sleep. Finally, 124 patients with moderate-to-severe AD in a Phase II randomized, double-blind, placebo-controlled study were instructed to take placebo, baricitinib 2 mg tablets, or baricitinib 4 mg tablets once daily for 16 weeks. All participants were allowed to apply topical triamcinolone. Compared to placebo, 61% of participants receiving 4 mg of baricitinib reported a 50% improvement in EASI at 16 weeks (placebo, 37%; P = 0.27). However, 57% of participants receiving 2 mg of baricitinib achieved EASI 50 compared to placebo (P = 0.65). One participant receiving baricitinib 2 mg discontinued treatment due to neutropenia, whereas five participants discontinued baricitinib 4 mg due to abnormal leukocyte counts, headache, and eczema. One participant receiving 4 mg of baricitinib reported a serious adverse event of a benign large intestine polyp.
Impact of atopic dermatitis
AD is a detrimental disease that significantly impacts the quality of life. It affects mental and physical functioning due to disease chronicity and sleep deprivation from pruritus., To explore the psychiatric impact from AD, a study of 380 AD patients reported anxiety symptoms more often than depression symptoms. Furthermore, 50% of participants lived with AD for more than 27 years, and 40% were diagnosed in adulthood.
In a study of 354,416 AD patients aged 2–17 years, AD was associated with increased rates of attention-deficit hyperactivity disorder diagnoses. In a study of AD patients aged 6–12 years, AD patients had higher levels of ADHD symptoms compared to control (OR: 1.88; 95% CI: 1.04–3.39). Pruritus is associated with sleep disturbances, psychological distress, and nocturnal scratching which causes daytime fatigue and impairment of daily activities.,,
Another study investigated the impact of AD on health-related quality of life and productivity in 349 AD patients and 698 controls. Among AD patients, 29.8% reported anxiety, 31.2% reported depression, and 33.2% reported sleep disorders compared to 16.1%, 17.3%, and 19.2% in the placebo group, respectively (all P < 0.001). Participants' responses to the standardized Short Form 36 questionnaire were used to measure their mental and physical health, reflected by the mental and physical component scores. Higher scores equated to better health status. The average mental component score in AD patients (44.5) was lower than controls (48.0; P < 0.001). The average Physical Component Score in AD patients (47.6) was lower than controls (49.5; P = 0.004). About 25.6% of AD patients self-reported an overall work impairment compared to 18.1% of controls (P = 0.004).
A prospective birth cohort study of 2916 participants reported AD patients with resolved AD in early childhood continued to have emotional and behavioral problems at 10 years of age. Based on the Strengths and Difficulties Questionnaire at 10 years of age, AD patients reported exacerbated mental health problems (OR: 1.49) and emotional symptoms (OR: 1.62).
| Discussion|| |
Epidermal dysfunction and immune dysregulation play key roles in the pathogenesis of AD. Other factors that contribute to AD pathogenesis include skin microbiota, sweat, and environment. Proteins that contribute to the epidermal barrier are downregulated in AD patients predisposing to inflammation and eczema formation.,, AD patients' susceptibility to S. aureus colonization and infection further decreases the integrity of the epidermal barrier. The FLG gene plays a role in AD pathogenesis. Loss of function in the FLG gene predisposes patients to more severe and persistent AD.,
Dupilumab is a promising new treatment option for AD patients who have exhausted topical medications. Phase II and Phase III trials demonstrated excellent improvement in AD skin and were well tolerated by patients.,
The JAK/STAT pathway is a promising therapeutic target in both topical and oral formulations. A 2% tofacitinib ointment applied twice daily improved EASI in 4 weeks. Furthermore, a Phase II study reported baricitinib as an effective treatment in adult participants with moderate-to-severe AD compared to placebo. Baricitinib and future JAK inhibitors are a promising treatment option in resistant AD cases limited to systemic therapy.
Research has demonstrated the impact of AD on quality of life. AD patients reported lower mental and physical scores compared to healthy controls (P < 0.001; P = 0.004). Adolescents diagnosed with AD and followed up at age 10 had increased mental health and emotional problems (OR: 1.49 and 1.62, respectively). Such findings increase the necessity for safe, effective, and affordable treatments.
| Conclusion|| |
Recent developments have increased understanding of the complexity and pathogenesis of AD. Newly approved medications have expanded the therapeutic options for patients and providers.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Harris VR, Cooper AJ. Atopic dermatitis: The new frontier. Med J Aust 2017;207:351-6.
Hanifin JM, Reed ML; Eczema Prevalence and Impact Working Group. A population-based survey of eczema prevalence in the United States. Dermatitis 2007;18:82-91.
Harrop J, Chinn S, Verlato G, Olivieri M, Norbäck D, Wjst M, et al.
Eczema, atopy and allergen exposure in adults: A population-based study. Clin Exp Allergy 2007;37:526-35.
Noda S, Krueger JG, Guttman-Yassky E. The translational revolution and use of biologics in patients with inflammatory skin diseases. J Allergy Clin Immunol 2015;135:324-36.
Shi B, Bangayan NJ, Curd E, Taylor PA, Gallo RL, Leung DY, et al.
The skin microbiome is different in pediatric versus adult atopic dermatitis. J Allergy Clin Immunol 2016;138:1233-6.
Gittler JK, Shemer A, Suárez-Fariñas M, Fuentes-Duculan J, Gulewicz KJ, Wang CQ, et al.
Progressive activation of T(H)2/T(H)22 cytokines and selective epidermal proteins characterizes acute and chronic atopic dermatitis. J Allergy Clin Immunol 2012;130:1344-54.
Williams MR, Gallo RL. The role of the skin microbiome in atopic dermatitis. Curr Allergy Asthma Rep 2015;15:65.
Dharmage SC, Lowe AJ, Matheson MC, Burgess JA, Allen KJ, Abramson MJ. Atopic dermatitis and the atopic march revisited. Allergy 2014;69:17-27.
Eckert L, Gupta S, Amand C, Gadkari A, Mahajan P, Gelfand JM. Impact of atopic dermatitis on health-related quality of life and productivity in adults in the United States: An analysis using the national health and wellness survey. J Am Acad Dermatol 2017;77:274-79.e3.
Tollefson MM, Bruckner AL, Section On Dermatology. Atopic dermatitis: Skin-directed management. Pediatrics 2014;134:e1735-44.
Kusari A, Han AM, Schairer D, Eichenfield LF. Atopic dermatitis: New developments. Dermatol Clin 2019;37:11-20.
Gavrilova T. Immune dysregulation in the pathogenesis of atopic dermatitis. Dermatitis 2018;29:57-62.
Hendricks AJ, Vaughn AR, Clark AK, Yosipovitch G, Shi VY. Sweat mechanisms and dysfunctions in atopic dermatitis. J Dermatol Sci 2018;89:105-11.
Kim HJ, Baek J, Lee JR, Roh JY, Jung Y. Optimization of cytokine milieu to reproduce atopic dermatitis-related gene expression in HaCaT keratinocyte cell line. Immune Netw 2018;18:e9.
Bao L, Mohan GC, Alexander JB, Doo C, Shen K, Bao J, et al.
A molecular mechanism for IL-4 suppression of loricrin transcription in epidermal keratinocytes: Implication for atopic dermatitis pathogenesis. Innate Immun 2017;23:641-7.
Danso M, Boiten W, van Drongelen V, Gmelig Meijling K, Gooris G, El Ghalbzouri A, et al.
Altered expression of epidermal lipid bio-synthesis enzymes in atopic dermatitis skin is accompanied by changes in stratum corneum lipid composition. J Dermatol Sci 2017;88:57-66.
Margolis DJ, Gupta J, Apter AJ, Ganguly T, Hoffstad O, Papadopoulos M, et al.
Filaggrin-2 variation is associated with more persistent atopic dermatitis in African American subjects. J Allergy Clin Immunol 2014;133:784-9.
Rupnik H, Rijavec M, Korošec P. Filaggrin loss-of-function mutations are not associated with atopic dermatitis that develops in late childhood or adulthood. Br J Dermatol 2015;172:455-61.
Palmer CN, Irvine AD, Terron-Kwiatkowski A, Zhao Y, Liao H, Lee SP, et al.
Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis. Nat Genet 2006;38:441-6.
Nowicka D, Grywalska E. The role of immune defects and colonization of Staphylococcus aureus
in the pathogenesis of atopic dermatitis. Anal Cell Pathol (Amst) 2018;2018:1956403.
Niebuhr M, Scharonow H, Gathmann M, Mamerow D, Werfel T. Staphylococcal exotoxins are strong inducers of IL-22: A potential role in atopic dermatitis. J Allergy Clin Immunol 2010;126:1176-83.e4.
Iwamoto K, Moriwaki M, Niitsu Y, Saino M, Takahagi S, Hisatsune J, et al. Staphylococcus aureus
from atopic dermatitis skin alters cytokine production triggered by monocyte-derived langerhans cell. J Dermatol Sci 2017;88:271-9.
Brunner PM, Leung DY, Guttman-Yassky E. Immunologic, microbial, and epithelial interactions in atopic dermatitis. Ann Allergy Asthma Immunol 2018;120:34-41.
Guttman-Yassky E, Krueger JG, Lebwohl MG. Systemic immune mechanisms in atopic dermatitis and psoriasis with implications for treatment. Exp Dermatol 2018;27:409-17.
Malajian D, Guttman-Yassky E. New pathogenic and therapeutic paradigms in atopic dermatitis. Cytokine 2015;73:311-8.
Brandt EB, Sivaprasad U. Th2 cytokines and atopic dermatitis. J Clin Cell Immunol 2011;2. pii: 110.
Hönzke S, Wallmeyer L, Ostrowski A, Radbruch M, Mundhenk L, Schäfer-Korting M, et al.
Influence of Th2 cytokines on the cornified envelope, tight junction proteins, and ß-defensins in filaggrin-deficient skin equivalents. J Invest Dermatol 2016;136:631-9.
Zheng T, Oh MH, Oh SY, Schroeder JT, Glick AB, Zhu Z. Transgenic expression of interleukin-13 in the skin induces a pruritic dermatitis and skin remodeling. J Invest Dermatol 2009;129:742-51.
Izuhara K, Nunomura S, Nanri Y, Ogawa M, Ono J, Mitamura Y, et al.
Periostin in inflammation and allergy. Cell Mol Life Sci 2017;74:4293-303.
Namkung JH, Lee JE, Kim E, Cho HJ, Kim S, Shin ES, et al.
IL-5 and IL-5 receptor alpha polymorphisms are associated with atopic dermatitis in Koreans. Allergy 2007;62:934-42.
D'Erme AM, Romanelli M, Chiricozzi A. Spotlight on dupilumab in the treatment of atopic dermatitis: Design, development, and potential place in therapy. Drug Des Devel Ther 2017;11:1473-80.
Furue M, Yamamura K, Kido-Nakahara M, Nakahara T, Fukui Y. Emerging role of interleukin-31 and interleukin-31 receptor in pruritus in atopic dermatitis. Allergy 2018;73:29-36.
Sgnotto FD, de Oliveira MG, Lira AA, Inoue AH, Titz TO, Orfali RL, et al.
IgG from atopic dermatitis patients induces IL-17 and IL-10 production in infant intrathymic TCD4 and TCD8 cells. Int J Dermatol 2018;57:434-40.
Kasraie S, Werfel T. Role of macrophages in the pathogenesis of atopic dermatitis. Mediators Inflamm 2013;2013:942375.
Marcinkiewicz M, Majewski S. The role of antimicrobial peptides in chronic inflammatory skin diseases. Postepy Dermatol Alergol 2016;33:6-12.
Di Meglio P, Perera GK, Nestle FO. The multitasking organ: Recent insights into skin immune function. Immunity 2011;35:857-69.
Jin S, Park CO, Shin JU, Noh JY, Lee YS, Lee NR, et al.
DAMP molecules S100A9 and S100A8 activated by IL-17A and house-dust mites are increased in atopic dermatitis. Exp Dermatol 2014;23:938-41.
Niyonsaba F, Kiatsurayanon C, Chieosilapatham P, Ogawa H. Friends or foes? Host defense (antimicrobial) peptides and proteins in human skin diseases. Exp Dermatol 2017;26:989-98.
Chieosilapatham P, Ogawa H, Niyonsaba F. Current insights into the role of human β-defensins in atopic dermatitis. Clin Exp Immunol 2017;190:155-66.
Howell MD, Fairchild HR, Kim BE, Bin L, Boguniewicz M, Redzic JS, et al.
Th2 cytokines act on S100/A11 to downregulate keratinocyte differentiation. J Invest Dermatol 2008;128:2248-58.
Wang R, Braughton KR, Kretschmer D, Bach TH, Queck SY, Li M, et al.
Identification of novel cytolytic peptides as key virulence determinants for community-associated MRSA. Nat Med 2007;13:1510-4.
Nakamura Y, Oscherwitz J, Cease KB, Chan SM, Muñoz-Planillo R, Hasegawa M, et al. Staphylococcus
δ-toxin induces allergic skin disease by activating mast cells. Nature 2013;503:397-401.
Zollner TM, Wichelhaus TA, Hartung A, Von Mallinckrodt C, Wagner TO, Brade V, et al.
Colonization with superantigen-producing Staphylococcus aureus
is associated with increased severity of atopic dermatitis. Clin Exp Allergy 2000;30:994-1000.
Nakatsuji T, Chen TH, Two AM, Chun KA, Narala S, Geha RS, et al. Staphylococcus aureus
exploits epidermal barrier defects in atopic dermatitis to trigger cytokine expression. J Invest Dermatol 2016;136:2192-200.
Gonzalez ME, Schaffer JV, Orlow SJ, Gao Z, Li H, Alekseyenko AV, et al.
Cutaneous microbiome effects of fluticasone propionate cream and adjunctive bleach baths in childhood atopic dermatitis. J Am Acad Dermatol 2016;75:481-93.e8.
Ono E, Murota H, Mori Y, Yoshioka Y, Nomura Y, Munetsugu T, et al.
Sweat glucose and GLUT2 expression in atopic dermatitis: Implication for clinical manifestation and treatment. PLoS One 2018;13:e0195960.
Sulzberger MB, Herrmann F, Zak FG. Studies of sweating; preliminary report with particular emphasis of a sweat retention syndrome. J Invest Dermatol 1947;9:221-42.
Papa CM, Kligman AM. Mechanisms of eccrine anidrosis. I. High level blockade. J Invest Dermatol 1966;47:1-9.
Murota H, Matsui S, Ono E, Kijima A, Kikuta J, Ishii M, et al.
Sweat, the driving force behind normal skin: An emerging perspective on functional biology and regulatory mechanisms. J Dermatol Sci 2015;77:3-10.
Krämer U, Lemmen CH, Behrendt H, Link E, Schäfer T, Gostomzyk J, et al.
The effect of environmental tobacco smoke on eczema and allergic sensitization in children. Br J Dermatol 2004;150:111-8.
Boguniewicz M, Leung DY. The ABC's of managing patients with severe atopic dermatitis. J Allergy Clin Immunol 2013;132:511-2.e5.
Henderson RL, Fleischer AB Jr., Feldman SR. Dermatologists and allergists have far more experience and use more complex treatment regimens in the treatment of atopic dermatitis than other physicians. J Cutan Med Surg 2001;5:211-6.
Cork MJ, Britton J, Butler L, Young S, Murphy R, Keohane SG. Comparison of parent knowledge, therapy utilization and severity of atopic eczema before and after explanation and demonstration of topical therapies by a specialist dermatology nurse. Br J Dermatol 2003;149:582-9.
Saeki H, Nakahara T, Tanaka A, Kabashima K, Sugaya M, Murota H, et al.
Clinical practice guidelines for the management of atopic dermatitis 2016. J Dermatol 2016;43:1117-45.
Paller AS, Tom WL, Lebwohl MG, Blumenthal RL, Boguniewicz M, Call RS, et al.
Efficacy and safety of crisaborole ointment, a novel, nonsteroidal phosphodiesterase 4 (PDE4) inhibitor for the topical treatment of atopic dermatitis (AD) in children and adults. J Am Acad Dermatol 2016;75:494-503.e6.
Hamilton JD, Suárez-Fariñas M, Dhingra N, Cardinale I, Li X, Kostic A, et al.
Dupilumab improves the molecular signature in skin of patients with moderate-to-severe atopic dermatitis. J Allergy Clin Immunol 2014;134:1293-300.
Malik K, Heitmiller KD, Czarnowicki T. An update on the pathophysiology of atopic dermatitis. Dermatol Clin 2017;35:317-26.
Chang HY, Nadeau KC. IL-4Rα inhibitor for atopic disease. Cell 2017;170:222.
Boguniewicz M. Biologic therapy for atopic dermatitis: Moving beyond the practice parameter and guidelines. J Allergy Clin Immunol Pract 2017;5:1477-87.
Hajar T, Gontijo JR, Hanifin JM. New and developing therapies for atopic dermatitis. An Bras Dermatol 2018;93:104-7.
Simpson EL, Gadkari A, Worm M, Soong W, Blauvelt A, Eckert L, et al.
Dupilumab therapy provides clinically meaningful improvement in patient-reported outcomes (PROs): A phase IIb, randomized, placebo-controlled, clinical trial in adult patients with moderate to severe atopic dermatitis (AD). J Am Acad Dermatol 2016;75:506-15.
Simpson EL, Bieber T, Guttman-Yassky E, Beck LA, Blauvelt A, Cork MJ, et al.
Two phase 3 trials of dupilumab versus placebo in atopic dermatitis. N
Engl J Med 2016;375:2335-48.
Tsianakas A, Ständer S. Dupilumab: A milestone in the treatment of atopic dermatitis. Lancet 2016;387:4-5.
Beck LA, Thaçi D, Hamilton JD, Graham NM, Bieber T, Rocklin R, et al.
Dupilumab treatment in adults with moderate-to-severe atopic dermatitis. N
Engl J Med 2014;371:130-9.
Thaçi D, Simpson EL, Beck LA, Bieber T, Blauvelt A, Papp K, et al.
Efficacy and safety of dupilumab in adults with moderate-to-severe atopic dermatitis inadequately controlled by topical treatments: A randomised, placebo-controlled, dose-ranging phase 2b trial. Lancet 2016;387:40-52.
Ou Z, Chen C, Chen A, Yang Y, Zhou W. Adverse events of dupilumab in adults with moderate-to-severe atopic dermatitis: A meta-analysis. Int Immunopharmacol 2018;54:303-10.
Saeki H, Kabashima K, Tokura Y, Murata Y, Shiraishi A, Tamamura R, et al.
Efficacy and safety of ustekinumab in Japanese patients with severe atopic dermatitis: A randomized, double-blind, placebo-controlled, phase II study. Br J Dermatol 2017;177:419-27.
Ruzicka T, Hanifin JM, Furue M, Pulka G, Mlynarczyk I, Wollenberg A, et al.
Anti-interleukin-31 receptor A antibody for atopic dermatitis. N
Engl J Med 2017;376:826-35.
Kim DH, Park KY, Kim BJ, Kim MN, Mun SK. Anti-immunoglobulin E in the treatment of refractory atopic dermatitis. Clin Exp Dermatol 2013;38:496-500.
Iribarren C, Rahmaoui A, Long AA, Szefler SJ, Bradley MS, Carrigan G, et al.
Cardiovascular and cerebrovascular events among patients receiving omalizumab: Results from EXCELS, a prospective cohort study in moderate to severe asthma. J Allergy Clin Immunol 2017;139:1489-95.e5.
Bissonnette R, Papp KA, Poulin Y, Gooderham M, Raman M, Mallbris L, et al.
Topical tofacitinib for atopic dermatitis: A phase IIa randomized trial. Br J Dermatol 2016;175:902-11.
Cotter DG, Schairer D, Eichenfield L. Emerging therapies for atopic dermatitis: JAK inhibitors. J Am Acad Dermatol 2018;78:S53-62.
Levy LL, Urban J, King BA. Treatment of recalcitrant atopic dermatitis with the oral janus kinase inhibitor tofacitinib citrate. J Am Acad Dermatol 2015;73:395-9.
Guttman-Yassky E, Silverberg JI, Nemoto O, Forman SB, Wilke A, Prescilla R, et al.
Baricitinib in adult patients with moderate-to-severe atopic dermatitis: A phase 2 parallel, double-blinded, randomized placebo-controlled multiple-dose study. J Am Acad Dermatol 2019;80:913-21.e9.
Simpson EL, Bieber T, Eckert L, Wu R, Ardeleanu M, Graham NM, et al.
Patient burden of moderate to severe atopic dermatitis (AD): Insights from a phase 2b clinical trial of dupilumab in adults. J Am Acad Dermatol 2016;74:491-8.
Strom MA, Fishbein AB, Paller AS, Silverberg JI. Association between atopic dermatitis and attention deficit hyperactivity disorder in U.S. Children and adults. Br J Dermatol 2016;175:920-9.
Schmitt J, Buske-Kirschbaum A, Tesch F, Trikojat K, Stephan V, Abraham S, et al.
Increased attention-deficit/hyperactivity symptoms in atopic dermatitis are associated with history of antihistamine use. Allergy 2018;73:615-26.
Yu SH, Attarian H, Zee P, Silverberg JI. Burden of sleep and fatigue in US adults with atopic dermatitis. Dermatitis 2016;27:50-8.
Silverberg JI, Garg NK, Paller AS, Fishbein AB, Zee PC. Sleep disturbances in adults with eczema are associated with impaired overall health: A US population-based study. J Invest Dermatol 2015;135:56-66.
Schmitt J, Apfelbacher C, Chen CM, Romanos M, Sausenthaler S, Koletzko S, et al.
Infant-onset eczema in relation to mental health problems at age 10 years: Results from a prospective birth cohort study (German infant nutrition intervention plus). J Allergy Clin Immunol 2010;125:404-10.
Vakharia PP, Chopra R, Sacotte R, Patel KR, Singam V, Patel N, et al.
Burden of skin pain in atopic dermatitis. Ann Allergy Asthma Immunol 2017;119:548-52.e3.